hHbA1c is prepared from lysed human blood cells by sequential ion-exchange chromatographic methods. Essentially free of HbA1a, HbA1b and HbA0a0, the purified protein is valedated by HPIEC methods and carbohydrate analysis, and is suitable as a reference material for hHbA1c testing. The reported concentration is determined by Drabkins assay. The material is supplied in liquid form in dilute potassium cyanide-phosphate buffer. The concentration varies with manufacturing lot, but the protein may be used directly by dilution in buffer in most experimental methods. It is shipped frozen at -20oC.
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Last Updated : 03/16/2013