PAPANICOLAOU STAINING SOLUTIONS
Company : ENG Scientific Inc
UMDC Code : 233277
Smears are alcohol-ether fixed. Smears are brought to distilled water through alcohols. Stain 2 - 3 minutes in Harris or Mayers Hematoxylin. Place in distilled water for 10 seconds. Rinse gently to prevent cells from being washed off (about 5 to 6 dips). Wash under running tap water for 6 minutes. Rinse in distilled water and transfer through graded alcohols; 10 seconds with dipping agitation in 50%, 70%, 80% and 95% alcohol. Stain in OG 6 solution for 1.5 minutes. Rinse smear dipping for 10 seconds in two individual changes of ethanol. If mucus is present, stain 1.5 minutes in EA 36, EA 50 or EA 65. In each of 3 baths of 95% alcohol, rinse for 10 seconds. In each of 2 baths of absolute ethyl alcohol, rinse for 10 seconds. Clear in 3 - 4 changes of xylene.
Product URL : http://www.engscientific.com/cyto.html
Last Updated : 03/16/2013