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Detection of antibodies against dsDNA.
Indication: lupus erythematosus disseminatus.
Initial dilution: 1:10, conjugate class anti-human IgG, FITC-labelled.
Animal pathogenic haemoflagellates of Crithidia luciliae are used for the detection of autoantibodies against double-stranded, native DNA (dsDNA, nDNA) with indirect immunofluorescence. These protozoa possess a giant mitochondrion containing dsDNA (kinetoplast) that, in general, does not show any of the other antigens present in the cell nuclei. Antibodies reacting with the kinetoplast are therefore only directed against dsDNA.
For the dete
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